Actinomycetes species is widespread distribution and genetic manipulability. It plays a significant role in this microbial community. aime of the study is to investigate the potential of Actinomycetes, especially Streptomyces spp., isolated from fruit orchard in Muzaffarpur District, North Bihar, India. It focused on their ability to produce cellulase, xylanase, and amylase enzymes. This versatility makes them crucial contributors to the environmental processes of lignocellulose degradation, illustrates their potential. Out of 30 isolates, 15 exhibited significant enzymatic activity, particularly in cellulase production. These isolates, which displayed various colony colors ranging from dark brown to dark grey, were selected for further analysis. Following purification, enzymatic screening revealed that 15 isolates demonstrated the ability to secrete cellulase, lipase, and protease. From these, 2 (SGIITD04 and SGIITD07) of the most promising isolates were identified as Streptomyces spp. through 16S rRNA sequencing. This study underscores the enzymatic potential of Streptomyces spp., particularly in cellulase production, exhibit their importance in lignocellulose degradation.