Actinomycetes, a group of Gram-positive bacteria characterized by a high G, C content and play a vital role in nutrient cycling and decomposition of organic matter in soil. This study describes the screening of Actinmycetes to produce industrially important enzymes and their molecular characterization which are crucial for breaking down complex lignocellulosic compounds. The isolates were screened for all the three components of cellulase complex (endoglucanase, exoglucanase and β-glucosidase). These enzymes have significant industrial applications in particularly biofuel production, paper, pulp industries and agriculture. However, potential of actinomycetes from different environmental niches, such as the rhizospheric zone of litchi plants, remains underexplored. Methods : A total of 25 actinomycete isolates were obtained from soil samples and collected from 8 different litchi gardens of Muzaffarpur District of North Bihar. These isolates were screened on their morphological characteristics, followed by physiological, biochemical characteristics and 16S rRNA Phylogenetic analysis including assessments of cellulase and xylanase. The screening involved culturing the isolates on carboxymethyl cellulose (CMC) agar and observing the formation of clear zones, which indicate enzyme activity. Out of 30 actinomycete isolates, 10 showed cellulase activity, five exhibited amylase activity and two produced both enzymes and isolates identified as Streptomyces sp. SGIITD9, Streptomyces sp. SGIITD10. These cellulolytic strains demonstrated strong enzyme activity under submerged fermentation and shown their potential for low-cost industrial applications. Further isolation from diverse substrates is needed, as the number of enzyme-producing isolates was relatively low. Among the 10 isolates, 2 promising strains (SGIITD09 and SGIITD10) were selected and identified through their 16S rRNA sequence as Streptomyces spp., exhibiting excellent enzyme activity.