Makhana (Euryale ferox Salisb.) known as fox nut or Gorgon nut is the member of the family Nympheaceae. Effect of different concentration i.e., 0.1%, 0.01%, 0.001% and 0.0001% of Kinetin on protein concentration of makhana (Euryale ferox Salisb.) during fruit development was analyzed from flowering to maturity. On the basis of this study, in immature fruit (Normal control) the seeds were more proteinases in the control condition reaching up to an average of 20.29µg/mg as fruit and seed matured protein concentration was gradually decreased. After giving the treatment with kinetin in different concentration it was observed that at after treatment of 0.01% kinetin, seed and fruit had higher concentration protein.