Wetlands are treasure house of several medicinal plants. The commercial value of these plants still need special attention of local people to utilize these plants for curative purposes. These plants are very sensitive to save the normal Physico-chemical parameter of wetland. Slight alteration of the normal physico-chemical parameter of wetland may results in partial or complete disappearance of these plants. The loss of these medicinal plants will also result in the traditional knowledge of their medicinal properties as well as economic loss. Thus in this study, an attempt has been made to document the medicinal plants of aquatic macrophytes wetlands of Laokhowa wildlife sanctuary, Nagaon, Assam.